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Using modern methods such as endolysosomal patch-clamp electrophysiology and the measurement of lysosomal calcium release via fluorescence microscopy, the researchers established that there was indeed an interaction between Rab7a and TPC2 at the functional level, which promoted the growth and invasiveness of melanoma cells.

“From a concerning standpoint, any time people have these types of rhythms their heart rates kind of spike up,” said Dr. Nikhil Warrier, medical director of electrophysiology at MemorialCare Heart & Vascular Institute at Orange Coast Medical Center in Fountain Valley.

The study is also the first to combine the use of two investigative techniques called electrophysiology and optogenetics to study this illusion.

"To really understand what goes on inside the brain during perceptual experiences, we need to use certain methods that we cannot use on people. These include electrophysiology, the recording of neural activity with electrodes, and optogenetics, where light pulses enable or disable firing of specific neurons in a living brain."

Watanabe's experiment was the first of its kind to make use of both electrophysiology and optogenetics at the same time in animal test subjects exposed to the neon-color-spreading illusion, which allowed his team to see precisely what structures within the brain are responsible for processing the illusion.